Protein biochemistry

Methods

We produce proteins on a laboratory scale. Our scientific and technical expertise comprises the full working cycle from the sequencing to the high-purity functional protein:

• Molecular biological work to generate expression vectors in accordance with the protein’s properties
• Expression of the proteins in various cell systems (E. coli, yeast, mammalian cells, insect cells) using the batch method or FED batch fermentation (5l scale)
• Purification via a range of chromatographic methods (affinity chromatography, hydrophobic interaction chromatography, size exclusion chromatography, ion exchange chromatography, reversed phase chromatography)
• Associated protein analytics for quality assurance 

• Based on spectrophotometric, fluorometric or fluorescence polarisation measurements in the 96 or 384 well format
• Parameters, such as K_m, k_cat, K_i, IC₅₀
• Examinations on the inhibition mechanism of inhibitors
• Determination of substance solubility using nephelometry

• Analysis of amyloidogenic proteins using fluorometric methods (Thioflavin T-assay)
• Statistical analysis of aggregation kinetics (to determine the lag-phase time and aggregation rate)
• Aggregation visualisation (together with cooperation partners) via transmission electron microscopy (TEM) and atomic force microscope (AFM)
• Analyses of the inhibition of aggregation by various agents (antibodies, small molecules, plant / bacterial extracts)