Fraunhofer Institute for Cell Therapy and Immunology IZI
In Vivo Models
The In Vivo Models Unit investigates active agents as well as cell and gene therapeutics with the aim of elucidating clinically relevant immunological issues in the living organism. One of the unit’s main areas of competence involves establishing and validating new preclinical animal models, especially in the field of hematology and immuno-oncology.
Established in vivo models
- Leukemia and lymphoma models in NSG and wild-type mice (acute myeloid leukemia (AML), mastocytoma, B-cell lymphoma)
- Xenogeneic, acute GvHD model in NSG mice
- Acute GvHD transplantation model (full haplotype mismatch) in wild-type mice (e.g. BALB/c or C3H/HeJ strain) or transgenic mice (huCD4+, muCD4-, HLA-DR3+)
- Xenogeneic GvHD/GvL transplantation model in NSG mice
- Preclinical investigation of CAR immune cells in tumor models using NSG mice
- Xenogeneic murine tumor models (e.g. AML, glioblastoma)
- Humanization of NSG mice
Service portfolio
- Establishing new in vivo models
- Designing animal experiments including sophisticated scoring matrices
- Writing applications to enable the conduct of animal experiments
- Consulting with animal welfare officers and communicating with the local authority
Methods and analyses
- In vivo bioluminescence and fluorescence measurements
- Small animal magnetic resonance imaging and computer tomography
- Total body and precise irradiation using X-ray equipment
- Blood analyses (hemogram, flow cytometry, clinical parameters)
- Histology and immunohistochemistry