Next-generation sequencing

For the control of quantity, quality and integrity of RNA and DNA samples, which can be isolated from various starting materials, the following equipment is available:

• Fluorometer (Qubit und DeNovix)
• Nanodrop 1000
• BioAnalyzer
• LightCycler® 480

Three different instruments are available for performing next-generation sequencing. Depending on the experimental requirements, they allow a flexible number of samples as well as a scalable data output.

The institute has its own high-performance computing cluster for data analysis.

• High Performance Computing Cluster

We use the platforms from 10x Genomics for cell separation, barcoding, and tissue transfer.:

• 10x Chromium Controller
• 10x Genomics Chromium Controller iX
• 10x CytAssist

• LUNA Automated Cell Counter
• Rotary microtome