Fraunhofer Institute for Cell Therapy and Immunology IZINext-Generation Diagnostics
High-resolution NGS methods
Single cell sequencing and multi-omics (10x GENOMICS workflows):
- Generation of transcriptome profiles and parallel detection of surface proteins at single cell level
- Application examples: Functional control of therapeutic CARs (e.g. CAR-T cells, CAR-NK cells) and monitoring the success of therapy in patients
- Available assays:
- Chromium GEM-X Single Cell 3' Gene Expression
- Chromium GEM-X Single Cell 5' Gene Expression
- Single Cell Immune Profiling
- Chromium Single Cell V(D)J (T-cell and/or B-cell receptor analysis is performed in combination with 5' gene expression analysis)
- Expression of surface proteins
Spatial sequencing (Visium Spatial10x GENOMICS):
- Spatially resolved gene expression profiles in tissues down to single cell resolution
- Application example: Parallel molecular and imaging characterization of tumor, tumor-infiltrating immune cells and tumor microenvironment
- Available assays:
- HD Spatial Gene Expression (FFPE tissue)
- Spatial Gene Expression (FFPE and cryogenic tissue)
Quality assurance
- ISO 9001 certified quality management system
- NGS and bioinformatics according to established SOPs
- DSGVO-compliant data processing
Available equipment / devices
- 10x Genomics Chromium Controller
- NextSeq 2000, Illumina
- MiSeq, Illumina
- MinION, Oxford Nanopore Technologies
- High Performance Computing Cluster
- LUNA Automated Cell Counter
- BioAnalyzer