Droplet digital PCR system
The droplet PCR system can be used for the PCR-based, absolute quantification of microbial / viral or eukaryotic DNA and RNA and is particularly suited to the precise detection of low genome copy numbers.
The system's two hardware components are the droplet generator, which produces the droplets, and the droplet reader, which analyses the droplets following successful PCR. Amplification takes place in approx. 20,000 nanoliter-sized drops, meaning that any existing target DNA molecules are separated from each other and detected during the fluorescence-based selection process. In determining the endpoint, a positive or negative signal is measured and counted drop by drop. The system can use intercalating dyes and also probes (for Fam/Vic dyes) and is especially suited to the investigation of biomarkers, infectious diseases and gene expression.
- up to 96 reactions/run
- 20 µl reaction volume
Fraunhofer IZI, main building, 2nd floor, B2 laboratory
Person responsible for equipment / contact person
Dr. Natalia Sandetskaya
Department of Diagnostics
Phone +49 341 35536-9310