Optical Coherence Tomography
Optical coherence tomography (OCT) is a highly sensitive, 3D imaging technology. It uses near-infrared light to depict the internal and surface structures of various materials in high resolution.
As opposed to x-ray tomography, neither the examination object nor the source detector array has to be rotated as the OCT procedure facilitates scanning. The light scattered from the sample is recorded at individual points; each measuring point holds information about the scattering properties inside the examined object (depth scattering profile). A volumetric recording of the examination object is made possible using a raster.
The system deployed in the BNAL impresses here with its high measuring speed of 76,000 interferometric measurements per second. This means that biological samples can be recorded three-dimensionally with a resolution in the range of a few thousandths of a millimeter in just seconds. At the same time, the use of low-energy, near-infrared radiation prevents biological tissue from being influenced in any way.
Following the addition of a respective automation system, the systematic and automated recording of different cell cultures, e.g. in multi-well plates, is possible in the BNAL using OCT.
- Measuring range: 10 x 10 x 7 mm³
- Measuring speed: 76 kHz
- Depth resolution (in air): 12 µm
- Scan resolution: 13 µm
Person responsible for equipment / contact person
Dipl.-Ing. (FH) Christian Wolf
Department of Bio- and Nanotechnology
Optical Coherence Tomography Unit
Phone +49 351 88815-618
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