Protein Misfolding Diseases

Methods

  • Production (cloning) of expression vectors
  • Heterologous expression of proteins in E. coli, yeast, and insect and mammalian cells
  • Column chromatographic purification of proteins on an analytical and semi-preparative scale
  • Spectroscopic analysis of protein structures and aggregation kinetics in vitro (CD and fluorescence spectroscopy)
  • Development of enzyme assays
  • Structure-based optimization of antibodies (protein engineering)
  • Electron microscopy, dynamic light scattering or small-angle X-ray scattering (together with cooperation partners)