Fraunhofer Institute for Cell Therapy and Immunology IZIBiophysical analytics
Interaction analytics
- Assay development
- Development of SPR assays: Development (+ validation) of SPR-based assays for the determination of bonding properties – quality control (under GLP)
- SPR-in vitro diagnostics: Development (+ validation) of SPR-based assays for the quantification of biomolecules (e.g. antibodies) in biological matrices (under GLP)
- Analysis of proteins, peptides, small molecules
- SPR characterisation of interactions between biomolecules with regard to kinetic bonding profile, bonding strength and bond specificity
- SPR high-throughput screening: Screening of bonding partners (e.g. antibodies) in various matrices
- ITC – thermodynamic profiling: Determination of KD, ΔH, ΔS, N of bonding partners (in context with SAR and structural data)
- ITC – determination of active species: Determination of the share of active biomolecules (e.g. antibodies, enzymes) through ITC measurements
Quantification of biomolecules
- Assay development
- Development of ELISAs (customised): Development (+ validation) of ELISAs based on own or commercial antibodies for the quantification of biological, PK, PD markers in biological matrices
- TR fluorescence in vitro diagnostics: Development (+ validation) of assays for the quantification of molecules on the basis of TR fluorescence in biological matrices
- Protein, peptide, and small molecule analytics
- Antibody screening: Screening of antibodies using ELISA, e.g. to generate monoclonal antibodies
- Biomarker ELISA: Quantification of biological, PK, PD markers using commercial ELISAs or ELISAs developed in-house
- Antibody specificity: Determination of the specificity of antibodies (target vs. off-targets)