Mouse model for infectious disease (GLP-like / GLP)

Salmonella enterica causes various diseases including food poisoning and typhoid fever. We provide a mouse model of infectious disease using Salmonella enterica to test antibacterial agents and potential therapeutics. Furthermore, immunomodulatory / immunotoxic effects of test substances can be revealed by infecting mice with a sublethal dose of Salmonella enterica and simultaneous exposure to the test substance. Mice can also be infected with a median lethal (LD₅₀) infection dose to determine the impact of test substances on mortality. The model can thus be used as therapy and / or toxicity model.

Species

Mouse

Fields of application

• Diagnosis and therapy model for infections with Salmonella enterica
• Testing of antibacterial agents and potential therapeutics
• Testing of immunomodulatory / immunotoxic substances
• Evaluation of application/treatment protocols for therapeutic agents
• Efficacy and safety studies for drug approval (non-GLP and GLP)

Endpoints / outcome parameters

• Survival rate
• Cytokine / antibody status in serum
• Bacterial burden in peritoneum and different organs
• Phenotyping of infiltrating inflammatory cells in spleen, lymph nodes and peritoneum
• Functional activity of different immune cell subtypes (phagocytosis, NO production, cytokine / antibody response etc.)

Readout parameters

• Daily evaluation of clinical symptoms
• Periodic blood sampling
• Flow cytometric analysis / cell sorting / imaging flow cytometry
• ELISA / multiplex analysis
• Quantitative real-time PCR
• Histology
• Immunohistochemistry / immunofluorescence

Quality management and validation

• Internal quality management (certified GLP test facility)
• Use of reference compounds (standard-of-care therapies)
• Randomisation
• Blinded induction / data acquisition and analysis
• Biometric expertise