Experimental Imaging

Phenotyping biological samples using multiple imaging methods forms a core competence of preclinical research. This enables thorough depiction, from the smallest structures (cell organelles) right through to entire organ systems, both in spatial and temporal resolution (4D). Fraunhofer IZI has access to a comprehensive, state-of-the-art equipment pool that enables the acquisition and evaluation of various (also correlative) image data. Partners and customers are advised on biological, technical and economic matters and supported in carrying out and evaluating experiments. Furthermore, experimental procedures and equipment can be used, adapted and developed.

• Inverted microscope
• Fluorescence excitation
• Objectives: 4x / 0,13 NA; 10x / 0,3 NA; 20x / 0,45 NA; 40x / 0,6 NA; 60x / 1,4 NA Oil; 100x / 1,4 NA Oil

• Confocal Laser Scanning microsope
• Live cell imaging
• Fully automated Temperature and CO2 control
• Laser lines with wavelength:
  • Diode laser: 405 nm
  • Argon laser: 458 nm, 488 nm, 514 nm
  • 1st HeNe laser: 543 nm
  • 2nd HeNe laser: 633 nm
• Objectives: 10x / 0,3 NA; 20x / 0,4 NA; 20x / 0,8 NA; 40x / 0,6 NA; 40x / 1,1 NA Water; 40x / 1,3 NA Oil; 63x / 1,4 NA Oil; 100x / 1,4 NA Oil

• Inverted microscope
• Fluorescence excitation
• Live cell imaging
• Fully automated Temperature and CO2 control
• Objectives: 10x / 0,3 NA; 20x / 0,4 NA; 20x / 0,8 NA; 40x / 0,6 NA; 40x / 1,1 NA Water; 40x / 1,3 NA Oil; 63x / 1,4 NA Oil; 100x / 1,4 NA Oil

• Live cell imaging
• Fully automated temperature and CO2 control
• Phase contra
• Fluorescence excitation:
  • GFP: 441-481 nm
  • Green: 473-498 nm
  • Orange 524-550 nm
  • RFP: 567-607 nm
  • NIR: 648-674 nm
• Objectives: 4x, 10x, 20x
• Assays: ATP metabolism, apoptosis, cytotoxicity, proliferation, spheroid/organoid, cell killing, scratch-wound, …

• Upright microscope
• Laser microdissection (LMD)
• Objectives: 5x, 10x, 20x, 40x, 63x