Pharmacokinetic studies in RjHan:SD rats

Species

Rat

Fields of application

Catheter-implanted rats are used. One catheter is placed in the left carotid artery and one in the right common jugular vein. The vascular accesses are used to apply small molecules and for subsequent blood collection. Advantage of using rats is the determination of full PK profiles from single animals. Compound concentration is determined by LC-MS.

The model can be used for the following fields of application:

• ADME screening
• Pharmacokinetics
• Proof of concept

Endpoints / outcome parameter

• Different routes of application (i.v., p.o., s.c., i.p.)
• Pharmacokinetic parameters:
  • c_max [ng/ml]
  • t_max [h]
  • AUC [(ng*h)/ml]
  • t_1/2 [h]
  • F [%]

Readout parameter

• Blood collection
• Compound concentration determined by LC-MS

Quality management and validation

• Individual method development and validation for LC-MS
• Pharma-like documentation, e.g. by electronic laboratory notebook and compound management system
• Internal quality management
• GLP (if necessary)

References

Feng, J., Fitz, Y., Li, Y., Fernandez, M., Cortes Puch, I., Wang, D., et al. Catheterization of the Carotid Artery and Jugular Vein to Perform Hemodynamic Measures, Infusions and Blood Sampling in a Conscious Rat Model. J. Vis. Exp. (95), e51881, doi:10.3791/51881 (2015).

Weblink

• Methods of the Molecular Biotechnology Unit