Fraunhofer Institute for Cell Therapy and Immunology IZI
Fraunhofer Institute for Cell Therapy and Immunology IZI

Experimental autoimmune encephalomyelitis (EAE) in female SJL mice

Species

Mouse

Fields of application

EAE is induced by a single s.c. application of an emulsion containing Freund’s adjuvant, Proteolipidprotein 139-151(S) and desiccated Mycobacterium tuberculosis. On a clinical scale ranging from 0 to 5 EAE symptoms are assessed and body weight is monitored in a daily manner for at least thirty days.

The model can be used for the following fields of application:

  • Pharmacodynamics and pharmacokinetics
  • (Patho)-physiological processes
  • Therapeutic efficacy
  • Proof of concept

Endpoints / outcome parameter

  • Disease severity: Level of disease (mean maximum clinical score, mean and median clinical score), extent of disease (total score, area under the curve), incidence, mean day of onset (in vivo)
  • Body weight (in vivo)
  • Central nervous system demyelination (ex vivo)
  • Infiltration of immune cells into nervous tissue (ex vivo)
  • Cytokine production

Readout parameter

  • Clinical score
  • Body weight
  • Histology (Luxol fast blue and various classical histological stains)
  • Immunohistochemistry
  • ELISA, qRT-PCR

Quality management and validation

  • Controls
  • Randomisation
  • Allocation concealment after immunization
  • Blinded data collection and analysis
  • Biometric expertise
  • Internal quality management

Weblink

Serial coronal sections of the thoracic spinal cord from a mouse with a clinical score of 2.25. A: Luxol Fast Blue (LFB) stain. B: LFB + cresyl violet stain. C: LFB + hematoxylin and eosin stain. Blue = myelin, violet = cell nuclei, pink = cytoplasm. Arrow: demyelination in the white matter and infiltration of cells. Magnification: 10x. Scale bar: 300 µm.
© Fraunhofer IZI
Serial coronal sections of the thoracic spinal cord from a mouse with a clinical score of 2.25. A: Luxol Fast Blue (LFB) stain. B: LFB + cresyl violet stain. C: LFB + hematoxylin and eosin stain. Blue = myelin, violet = cell nuclei, pink = cytoplasm. Arrow: demyelination in the white matter and infiltration of cells. Magnification: 10x. Scale bar: 300 µm.