Translation-active lysates form the basis for the synthesis of proteins in cell-free systems. Endogenous microsomes in eukaryotic lysates are essential to guarantee a correct translocation in the microsomal membrane to display post-translational modified proteins such as glycoproteins and membrane-based proteins. The Eukaryotic Lysates Unit has many years of experience in the cultivation of eukaryotic cell lines and their conversion into translation-active lysate for the synthesis of proteins. The evaluation of a new cell line’s capacity for in-vitro expression is of great significance. The development and continuous optimisation of eukaryotic cell-free translation systems is one of the unit's important research areas. The influence on lysate productivity of fermentation, cell disruption, and transcription and translation components is of crucial interest.