Biomimetic Functional Materials

The Biomimetic Functional Materials Unit is concerned with technologies and solutions for rapid immuno- and binding assays. Since shelf-life and storage are of great significance for converting these highly specialised reagents into products, the unit places special emphasis on developing characteristics and processes for gentle drying and fixation in POCT systems. The field of “dry reagents” also applies to areas beyond analytical / diagnostic questions, for example for the development of pharmaceutical products and food additives.

  • Homogeneous immunoassays with electrochemical detection (proprietary format)
  • Customer-specific formulations of dry reagents and long-term stable reagents especially for applications in the POCT field (among others, the lyophilisation of antibodies, enzymes and chemicals)
  • Customer-specific synthesis of bioconjugates
  • Smart polymers (chemo-responsive polymers, redox polymers)
  • Development of molecular imprinted polymers (MIPs) for sensor systems and chromatography
  • Electrochemical investigation techniques

  • Lyophilisation facilities
  • Electrochemical workstations
  • Imaging ellipsometry (layer thicknesses, optical constants)
  • Quartz crystal micro-balance
  • High-speed video microscopy (up to 200,000 images/second)

Publications

  • Dechtrirat D, Gajovic-Eichelmann N, Bier FF, Scheller FW. Hybrid Material for Protein Sensing Based on Electrosynthesized MIP on a Mannose Terminated Self-Assembled Monolayer. Adv Func Mater, 24 (2014): 2233-2239.
  • Dechtrirat D, Gajovic-Eichelmann N, Wojcik F, Hartmann L, Bier FF, Scheller FW. Electrochemical displacement sensor based on ferrocene boronic acid tracer and immobilized glycan for saccharide binding proteins and E. Coli. Biosens Bioelectron. 58 (2014): 1-8.
  • Hüttl C, Hettrich C, Riedel M, Henklein P, Rawel H, Bier FF. Development of peptidyl lysine dendrons: 1,3-Dipolar cycloaddition for peptide coupling and antibody recognition. Chemical Biology and Drug Design. 2014 Oct 7. DOI dx.doi.org/10.1111/cbdd.12444.
  • Tanne J, Kracher D, Dietzel B, Schulz B, Ludwig R, Lisdat F, Scheller FW, Bier FF. Carboxylated or aminated polyaniline-multiwalled carbon nanotubes nanohybrids for immobilization of cellobiose dehydrogenase on gold electrodes. Biosensors. 2014 Oct 22;4(4):370-386. DOI dx.doi.org/10.3390/bios4040370.
  • Bosserdt M, Gajovic-Eichelmann N, Scheller FW. Modulation of direct electron transfer of cytochrome c by a molecularly imprinted thin film. Anal. Bioanal. Chem. (2013), 405(20):6437-6444.
  • Dechtrirat D, Jetzschmann K, Stoecklein WFM, Scheller FW, Gajovic-Eichelmann N. Protein Rebinding to a Surface-Confined Imprint. Adv Func Mater (2012), 22(24): 5231-5237.
  • Ettlinger J, Schenk JA, Micheel B, Ehrentreich-Förster E, Gajovic-Eichelmann N. A Direct Competitive Homogeneous Immunoassay for Progesterone – the Redox Quenching Immunoassay. Electroanalysis (2012), 24(7):1567 – 1575
  • Yarman A, Gröbe G, Neumann B, Kinne M, Gajovic-Eichelmann N, Wollenberger U, Hofrichter M, Ullrich R, Scheibner K, Scheller FW. The aromatic peroxygenase from Marasmius rutola--a new enzyme for biosensor applications. Anal Bioanal Chem. 2012 Jan;402(1):405-12.
  • Yarman A, Neumann B, Bosserdt M, Gajovic-Eichelmann N, Scheller FW. Peroxide-Dependent Analyte Conversion by the Heme Prosthetic Group, the Heme Peptide “Microperoxidase-11” and Cytochrome c on Chitosan Capped Gold Nanoparticles Modified Electrodes. Biosensors (2012), 2:189-204.
  • Yarman A, Nagel T, Gajovic-Eichelmann N, Fischer A, Wollenberger U, Scheller FW. Bioelectrocatalysis by Microperoxidase-11 in a multilayer architecture of chitosan embedded gold nanoparticles. Electroanalysis (2011), 23 (3), 611-618.
  • Yarman A, Badalyan A, Gajovic-Eichelmann N, Wollenberger U, Scheller FW. Enzyme electrode for aromatic compounds exploiting the catalytic activities of microperoxidase-11. Biosens Bioelectron., (2011), 30(1):320-323.
  • Schumacher S, Grüneberger F, Katterle M, Hettrich C, Hall DG, Scheller FW, Gajovic-Eichelmann N. Molecular imprinting of fructose using a polymerizable benzoboroxole: Effective complexation at pH 7.4. Polymer, Volume 52, Issue 12, 26 May 2011, Pages 2485-2491.
  • Schumacher S, Nagel T, Scheller FW, Gajovic-Eichelmann N. Alizarin Red S as an electrochemical indicator for saccharide recognition. Electrochim Acta (2011), 56(19):6607-6611. Schumacher S, Katterle M, Hettrich C, Paulke BR, Pal A, Hall DG, Scheller FW, Gajovic-Eichelmann N. Benzoboroxole-modified nanoparticles for the recognition of glucose at neutral pH. Chemical Sensors (2011), 1: 1-7.
  • Schumacher S, Katterle M, Hettrich C, Paulke BR, Hall DG, Scheller FW, Gajovic-Eichelmann N. Label-free detection of enhanced saccharide binding at pH 7.4 to nanoparticulate benzoboroxole based receptor units. J. Mol. Recognit. (2011); 24(6):953-9.
  • Athikomrattanakul U, Gajovic-Eichelmann N, Scheller FW. Thermometric sensing of nitrofurantoin by noncovalently imprinted polymers containing two complementary functional monomers. Anal. Chem. (2011), 83(20):7704-11.
  • Athikomrattanakul U, Katterle M, Gajovic-Eichelmann N, Scheller FW. Preparation and characterization of novel molecularly imprinted polymers based on thiourea receptors for nitrocompounds recognition. Talanta (2011), 84(2):274-9.
  • Tan C, Gajovic-Eichelmann N, Polzius R, Hildebrandt N, Bier FF. Direct detection of ?9-tetrahydrocannabinol in aqueous samples using a homogeneous increasing fluorescence immunoassay (HiFi). Anal Bioanal Chem. (2010); 398(5):2133-40.
  • Tan C, Gajovic-Eichelmann N, Stöcklein WF, Polzius R, Bier FF. Direct detection of Delta9-tetrahydrocannabinol in saliva using a novel homogeneous competitive immunoassay with fluorescence quenching. Anal Chim Acta. (2010); 658(2):187-92.
  • Nagel B, Gajovic-Eichelmann N, Scheller FW, Katterle M. Ionic topochemical tuned biosensor interface. Langmuir. (2010); 26(11):9088-93.
  • Athikomrattanakul U, Katterle M, Gajovic-Eichelmann N, Scheller FW. Development of molecularly imprinted polymers for the binding of nitrofurantoin. Biosens Bioelectron. (2009); 25(1):82-7.
  • Nagel T, Gajovic-Eichelmann N, Tobisch S, Schulte-Spechtel U, Bier FF. Serodiagnosis of Lyme borreliosis infection using surface plasmon resonance. Clin Chim Acta. (2008); 394(1-2):110-3.
  • Schwonbeck S, Krause-Griep A, Gajovic-Eichelmann N, Ehrentreich-Förster E, Meinl W, Glatt H, Bier FF. Cohort analysis of a single nucleotide polymorphism on DNA chips. Biosens Bioelectron. (2004); 20(5):956-66.
  • Gajovic-Eichelmann N, Ehrentreich-Förster E, Bier FF. Directed immobilization of nucleic acids at ultramicroelectrodes using a novel electro-deposited polymer. Biosens Bioelectron. (2003);19(5):417-22.
  • Hölzel R, Gajovic-Eichelmann N, Bier FF. Oriented and vectorial immobilization of linear M13 dsDNA between interdigitated electrodes – towards single molecule DNA nanostructures. Biosens Bioelectron. (2003);18(5-6):555-64.

Patents

  • Ettlinger J, Gajovic-Eichelmann N, Micheel B, Scharte G, Schenk J. Method for electrochemical detection of binding reactions. WO 2011/147563 A1. 1 Dec. 2011.
  • Athikomrattanakul U, Gajovic-Eichelmann N. Polymer matrix with target binding site for creatinine and derivatives thereof, its preparation and uses. WO 2013/170914 A1. 21 Nov. 2013.