Imaging and image evaluation

Phenotyping biological samples using multiple imaging methods forms a core competence of preclinical research. This enables thorough depiction, from the smallest structures (cell organelles) right through to entire organ systems, both in spatial and temporal resolution (4D). Fraunhofer IZI has access to a comprehensive, state-of-the-art equipment pool that enables the acquisition and evaluation of various (also correlative) image data. Partners and customers are advised on biological, technical and economic matters and supported in carrying out and evaluating experiments. Furthermore, experimental procedures and equipment can be used, adapted and developed.

Bruker PharmaScan 7T

  • Magnetic resonance imaging (MRI) of small animals
  • Different body-, head-, and surface coils
  • Anesthesia device
  • ECG, respiration, and temperature control
  • Latest Software: Paravision 6.0.1
  • Simple access to the Center for Experimental Medicine
Magnetic resonance imaging
© Fraunhofer IZI

Magnetic resonance imaging

Xstrahl SARRP

  • Computed tomography (CT) for small animals
  • Radio therapy (RT)
  • Direct access to the Center for Experimental Medicine
Xstrahl SARRP
© Fraunhofer IZI

Xstrahl SARRP

XENOGEN IVIS Spectrum

  • Bioluminescence Imaging (BLI) and Fluorescence Imaging (FLI)
  • Wide spectral range from 500 nm to  900 nm
  • Anesthesia device for five animals
  • Simple access to the Center for Experimental Medicine
XENOGEN IVIS Spectrum
© Fraunhofer IZI

XENOGEN IVIS Spectrum

Bedside imaging for small animals

  • Various ultrasound units with a number of transducers and an implemented Color Doppler
  • Flexible miniature cameras for the routine endoscopic examination of small animals and for the development of new lens attachments

Nikon Eclipse Ti

  • Inverted microscope
  • Objectives: 4x, 10x, 20x, 40x, 60x Oil, 100x Oil
  • Fluorescence excitation
Nikon Eclipse Ti
© Fraunhofer IZI

Nikon Eclipse Ti

Zeiss Observer.Z1 with incubation chamber

  • Inverted microscope
  • Fluorescence excitation
  • Live cell imaging
  • Fully automated Temperature and CO2 control
  • Objectives: 1x 0,025 Air, 10x 0,3 Air 20x 0,5 Air, 20x 0,4 Air PH2, 20x 0,8 Air, 40x 0,6 Air PH2, 40x 0,75 Air, 40x 1,1 Water Corr, 40x 1,3 Oil DIC, 63x 1,4 Oil DIC, 100x 1,4 Oil
Zeiss Observer.Z1 with incubation chamber
© Fraunhofer IZI

Zeiss Observer.Z1 with incubation chamber

Zeiss Observer.Z1 with LSM 710

  • Confocal Laser Scanning microsope
  • Live cell imaging
  • Laser lines with wavelength:
    • Diode laser: 405 nm
    • Argon laser: 458 nm, 488 nm, 514 nm
    • 1st HeNe laser: 543 nm
    • 2nd HeNe laser: 633 nm
Zeiss Observer.Z1 with LSM 710
© Fraunhofer IZI

Zeiss Observer.Z1 with LSM 710

Zeiss Imager.D1 with MBF Stereo Investigator

  • Upright microscope for stereology
  • Stereo Investigator from MBF biosciences
  • Fluorescence excitation
  • Objectives: 1x 0,25 Air, 10x 0,3 Air 20x 0,5 Air, 20x 0,4 Air PH2, 20x 0,8 Air, 40x 0,6 Air PH2, 40x 0,75 Air, 40x 1,1 Water Corr, 40x 1,3 Oil DIC, 63x 1,4 Oil DIC, 100x 1,4 Oil
Zeiss Imager.D1 with MBF Stereo Investigator
© Fraunhofer IZI

Zeiss Imager.D1 with MBF Stereo Investigator

Leica LMD 7000

  • Laser microdissection (LMD)
  • Upright microscope
  • Objectives: 5x, 10x, 20x, 40x, 63x
Leica LMD 7000
© Fraunhofer IZI

Leica LMD 7000

The Image Analysis of Cell Function Unit develops new, bespoke image analysis methods for the non-destructive, microscopy-based quantification of physiological and pathological processes.

By analyzing cells and tissue without modifying or destroying them, the unit aims to support research into fundamental biological relationships as well as the testing of new therapeutic methods. As this requires interdisciplinary cooperation in the fields of electrical engineering, optics, imaging, software development and biology, the specialist group is closely tied in to the Biotronic Systems chair at Leipzig University of Applied Sciences (HTWK).

In histology and pathology, immunohistochemical sections are still indispensable. In order to evaluate histological sections according to high standards, they are being increasingly digitalized and examined with complex image analysis tools. Our institute is intensively researching the development of these techniques and offers slide scanning services and professional image analysis.

Our offer

Slide scanning:

  • Scan the slides
  • Online access to the digital slides available
  • Shipment of the digital slides on a data storage medium
  • Slide scanning under GLP conditions

 

In addition to the slide scanning services, we offer
Professional image analysis:

  • Image analysis by experts according to your needs

Advantages

  • Slide scanning and image analysis in one step
  • High standards and high image quality
  • Tailored image analysis
  • Image analysis by our imaging experts
  • Access the data online or on a data carrier
  • Constant improvement of our analysis tools by our engineers