ivD Platform / PoC Technologies

The unit develops processes and devices for highly diverse point-of-care applications. An in vitro diagnostics (ivD) platform is available for this purpose, which can be adapted to various diagnostic tests depending on the topic of study.

The Fraunhofer ivD platform consists of a cartridge the size of a credit card (Lab on-a-chip) and a base station. Blood or other biological liquids can be applied directly on the cartridge and subsequently analysed automatically in the base station within 10–15 minutes. Using microarrays, a multiparameter diagnostic method is possible by employing various capture molecules such as antibodies, proteins, peptides, glycane or oligonucleotides.

Apart from the development of new diagnostic processes, the unit offers customers and partners the transfer of existing tests (e.g. ELISAs, DNA microarrays, …) to the ivD platform, including optimisation and technical verification up to ivD approval.

  • Assay development for serological assays
  • Assay development for blood, urine, saliva samples and other media
  • Multiplex assays for up to 500 analytes
  • DNA-based assays (microarrays)
  • PCR and RT-PCR development
  • Antibody assays
  • Dispensation of diverse biological samples in various volumes/viscosities
  • Coupling of DNA, RNA, proteins and peptides to modified surfaces, (glass, gold, silicon, polymer substrates etc.)
  • Aptamers for in-vitro diagnostics
  • Biomolecular interaction analysis, kinetic analysis
  • Kinetic ranking of interactions by means of a Biacore-Flex chip (peptide antibodies, protein DNA etc.)
  • Characterisation and validation of antibodies (cross-reactivity, specificity)
  • Serum screening
  • Aptamer assays
  • Assay development for biochips, microarray-based assays
  • Feasibility studies for alternative detection systems

  • S1/S2 laboratories, assay development for pathogen detection
  • Multiplex assays
  • Microarray facilities, spotting: biochip arrays for creating biochips (Genpix, non-contact, Scienion S5 & S7, TopSpotter, i2 from M2)
  • Scanner technology (Axon, Tecan)
  • Contact-free biosensors (Biacore Flexchip, T100)
  • Fluorescence MTP reader
  • Nanoliter micro-dispenser
  • Hybridisation station for semi-automatic microarray processing

  • Bader D, Klier DT, Hettrich C, Bier FF, Wessig P. Detecting carbohydrate–lectin interactions using a fluorescent probe based on DBD dyes. Anal. Methods. 2016 Jan 13; 8, 1235-1238: DOI dx.doi.org/10.1039/C5AY02991K
  • Hoppe S, Bier FF, von Nickisch-Rosenegk M. Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae. PLoS One 9(10): e110703. Doi dx.doi.org/10.1371/journal.pone.0110703.
  • Scheller FW, Yarman A, Bachmann T, Hirsch T, Kubick S, Renneberg R, Schumacher S, Wollenberger U, Teller C, Bier FF. Future of Biosensors: A Personal View. Adv Biochem Eng Biotechnol. 140 (2014):1-28.
  • Kozma P, Kehl F, Ehrentreich-Förster E, Stamm C, Bier FF. Integrated planar optical waveguide interferometer biosensors: A comparative review. Biosens. Bioelectron. 58 (2014):287-307.
  • Kersting S, Rausch V, Bier FF, von Nickisch-Rosenegk M. Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis. Malaria J., 13 (2014):99. (e1-e9).
  • Kersting S, Rausch V, Bier FF, von Nickisch-Rosenegk M. Multiplex isothermal solid-phase recombinase polymerase amplification for the specific and fast DNA-based detection of three bacterial pathogens. Mikrochim Acta. 181 (2014):1715-1723.
  • Bier FF, Schumacher S. Integration in Bioanalysis: Technologies for Point-of-Care Testing. Adv Biochem Eng Biotechnol. 133 (2013): 1-14.
  • Kozma P, Lehmann A, Wunderlich K, Michel D, Schu¬macher S, Ehrentreich-Förster E, Bier FF. A novel handheld fluorescent microarray reader for point-of-care diagnostic. Biosensors and Bioelectronics 47 (2013): 415–420.
  • Schumacher S, Lüdecke C, Ehrentreich-Förster E, Bier FF. Platform Technologies for Molecular Diagnostics Near the Patient's Bedside. Adv Biochem Eng Biotechnol. 133 (2013): 75-87.
  • Schumacher S, Sartorius D, Ehrentreich?Förster E, Bier FF. Miniaturization for Point-of-Care analysis: Platform technology for almost every biomedical assay. The Journal of the International Federation of Clinical Chemistry and Laboratory Medicine 23 (2012), 1-6.
  • Hoppe S, Bier FF, von Nickisch-Rosenegk M. Microarray-based method for screening of immunogenic proteins from bacteria. J Nanobiotechnology. 10 (2012): e12 (1-8).
  • von Nickisch-Rosenegk M, Teschke T, Bier FF. Construction of an artificial cell membrane anchor using DARC as a fitting for artificial extracellular functionalities of eukaryotic cells. J Nanobiotechnology 10 (2012): e1 (1-7).
  • Schumacher S, Nestler J, Otto T, Wegener M, Ehrentreich-Förster E, Michel D, Wunderlich K, Palzer S, Sohn K, Weber A, Burgard M, Grzesiak A, Teichert A, Brandenburg A, Koger B, Albers J, Nebling E, Bier FF. Highly-integrated lab-on-chip system for point-of-care multiparameter analysis. Lab Chip. 12 (2012): 464-473.
  • Peter H, Berggrav K, Thomas P, Pfeifer Y, Witte W, Templeton K, Bachmann TT. Direct detection and genotyping of Klebsiella pneumoniae carbapenemases from urine by use of a new DNA microarray test. J. Clin. Microbiol (2012) 50 (12), S. 3990–3998.
  • Reiss E, Hölzel R, Bier FF. Preparation of DNA nanostructures with repetitive binding motifs by rolling circle amplification. Methods Mol. Biol (2011) 749, S. 151–168.